Coding

Part:BBa_K1975003

Designed by: Joachim Steen Larsen   Group: iGEM16_UNIK_Copenhagen   (2016-06-28)


PhaB - NADPH-dependent acetoacetyl-CoA reductase

NADPH-dependent acetoacetyl-CoA reductase, or more commonly known as PhaB, is the second enzyme in the biological process of making the bioplastic polyhydroxybutyrate (PHB). This enzyme catalyzes the reduction from acetoacetyl-CoA to (R)-3-hydroxybutyrate-CoA. By combining this gene with LPE, which is a synthetic gene, it is possible to change the end-product from PHB to P(LA-co-3HB).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 697
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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